Dengue virus (DENV) is the world most prevalent mosquito-transmitted virus. The incidence of\ndengue infection has been increasing and most of the infected people are asymptomatic or have\nnon specific febrile illness. Laboratory test is essential to confirm dengue infection in epidemiological\nstudies. We developed an indirect ELISA test based on monoclonal immunoglobulin against\nall four DENV serotypes and evaluated the test for the diagnosis of asymptomatic dengue infection\nin paired annual serum samples. The indirect ELISA was found to have a sensitivity 87.5% and\nspecificity 78.3% at optimized cut off. The results from indirect ELISA demonstrate an incidence of\nasymptomatic dengue infection from children aged 4 - 11 years in Ratchaburi province, Thailand\n(2006-2009). These findings indicate that the indirect ELISA is suitable for serodiagnosis and seroepidemiological\nstudies of asymptomatic dengue infection.
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